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Western blot of the G5:MBP fusion expressed by the vector pMal-X. The blot was first probed with rabbit anti-MBP polyclonal Ab (A) and then stripped and reprobed with MAb loop2 (B). (Lane 1) Whole-cell extract harvested immediately prior to IPTG induction or (lane 2) four hours after IPTG induction. (Lane 3) Osmotic-shock supernatant containing processed, G5:MBP protein (indicated by arrow). (Lane 4) Resuspended osmotic-shock pellet containing mostly unprocessed protein.
