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SDS–PAGE showing the effect of the prlA4 mutation on periplasmic levels of the C6:MBP fusion in E. coli. Osmotic-shock supernatants containing C6:MBP fusion protein were simultaneously produced in prlA+ DH5αF′ (lane 2) and AR182 (prlA4) cells (lane 3). The sizes (in kDa) of the molecular weight standards (lane 1) are indicated (left), as is the mature C6:MBP fusion protein (arrowhead, right).
