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. 2014 Mar 17;204(6):891–900. doi: 10.1083/jcb.201305049

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© 2014 Nabti et al.

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Figure 3. — Inhibition of CDK1 and MAPK induces Mad2 dissociation from kinetochores. (A) Representative images of immunostaining for DNA, CREST, and Mad2 in nontreated control oocytes and oocytes treated with roscovitine, UO126, or both roscovitine and UO126. (B) Proportion of Mad2-positive kinetochores in control (n = 28), both roscovitine- and UO126 (R + U; n = 28)-, roscovitine (R; n = 31)-, and UO126 (U; n = 29)-treated oocytes. (C) Representative images of meiotic spindles from control nontreated meiosis I oocytes (n = 10) and oocytes treated with roscovitine and UO126 (n = 20). (D) Representative images of control nontreated meiosis I oocytes (n = 10) and oocytes treated with the Mps1 inhibitor AZ3146 (n = 10) immunostained for DNA, CREST, and Mad2. (E and F) Western blot (20 oocytes/lane; E) and analysis of securin (F) in oocytes at meiosis I in the presence or absence of AZ3146. In B and F, results were compared with the nontreated control or to each other where indicated and expressed as SEMs. NS, P > 0.05; *, P < 0.05; ***, P < 0.001. Bars, 5 µm.