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Surface plasmon resonance (SPR) sensorgrams and IC50 measurement of LMWH-II, enoxaparin and UFH using surface competition SPR. (a) Competition SPR sensorgrams of ATIII-heparin interaction (solution LMWH/surface heparin competition); (b) Competition SPR sensorgrams of PF4-heparin interaction (solution LMWH/surface heparin competition); (c) IC50 measurement for ATIII binding; (d) IC50 measurement for PF4 binding.
