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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Biopolymers. 2014 Jun;101(6):693–702. doi: 10.1002/bip.22441

FIGURE 5.

FIGURE 5

PLA assay to show HER2:HER3 heterodimerization and its inhibition by compound 21 in SKBR-3 cells. A) HER2:HER3 heterodimerization detected by PLA probe shown as red dots. B) SKBR-3 cells without any antibody to HER2 and HER3 and with PLA probes. There are no red dots, indicating the specificity of the assay. C) In the presence of control compound (CP), red dots are observed, suggesting that the control compound does not inhibit heterodimerization. D) in the presence of compound 21, the number of red dots decreased significantly compared to the control, suggesting the inhibition of HER2:HER3 heterodimerization.