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. 2014 Apr 15;9:1855–1870. doi: 10.2147/IJN.S51880

Figure 5.

Figure 5

Fluorescence and transmission light microscopy of anti-TEM1 Ab-/scFv-armed fluorescein- and shikonin-loaded PLGA NPs in endothelial MS1 cells.

Notes: (A) Untreated TEM1-negative MS1 cells. (B) Anti-TEM1 Ab-armed NPs treated TEM1-negative MS1 cells. (C) Anti-TEM1 scFv-armed NPs treated TEM1-negative MS1 cells. (D) Untreated TEM1-positive MS1 cells. (E) Anti-TEM1 Ab-armed NPs treated TEM1-positive MS1 cells. (F) Anti-TEM1 scFv-armed NPs treated TEM1-positive MS1 cells. Green color represents fluorescein-loaded PLGA NPs. (G–I), respectively, represent light microscopy, fluorescence microscopy, and light microscopy–fluorescence microscopy superimposed images of anti-TEM1 scFv-armed NPs treated TEM1-positive MS1 cells. Phalloidin–tetramethylrhodamine B isothiocyanate was used to stain the cytoplasmic F-actin (red). 4′,6-diamidino-2-phenylindole was used to stain the nucleus (blue).

Abbreviations: Ab, antibody; NPs, nanoparticles; PLGA, poly(lactic-co-glycolic acid); scFv, single-chain variable fragment; TEM1, tumor endothelial marker 1.