Table 3. Phenomic and modeling data from growth screens of G. metallireducens GS-15 wild type and dcuB with acetate as an electron donor.
Strain | Acceptor/Growth Mode (Donor Acetate) | Growth Rate (hr-1) | Donor Uptake Rate (mmol gDW-1 hr-1) | Acceptor Uptake Rate (mmol gDW-1 hr-1) | Acceptor/Donor Ratio |
GS-15 dcuB | Fumarate/Batch | 0.114±0.005 | 6.81±0.10 | 14.0±0.2 | 2.06±0.01 |
GS-15 dcuB | Fumarate/Chemostat* | 0.05 | 2.79±0.23 | N.D. | N.D. |
GS-15 | Nitrate/Batch∧ (byproduct ammonia) | 0.111±0.022 | 5.20±2.08 | 4.67±0.64 (no3) −3.98±0.38 (nh4) | 0.90±0.42 |
GS-15 | Fe(III)/Batch | N.D. | N.D. | N.D. | 6.96±0.43 |
GS-15 | Fe(III)/Chemostat* | 0.05 | 7.86±1.02 | 58.29±9.08 | 7.41±0.64 |
Simulation GS-15 dcuB | Max Growth Rate Fumarate | 0.116 | 6.22 | 14.2 (lim) | 2.28 |
Simulation GS-15 dcuB | Max Growth Rate Fumarate | 0.054 | 3.02 (lim) | 7.06 | 2.34 |
Simulation GS-15 | Max Growth Rate Nitrate | 0.118 | 7.28 (lim) | 4.35 −3.61 (lim) | 0.60 |
Simulation GS-15 | Max Growth Rate Fe(III) | 0.047 | 8.88 (lim) | 62.31 | 7.02 |
* Donor-limited chemostat;
N.D., Not Determined; lim, limiting rate in the simulation;
calculated error using a 90% confidence interval from Lovley and Phillips, 1988 [27].