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. 2014 Apr 24;10(4):e1004286. doi: 10.1371/journal.pgen.1004286

Figure 2. Four of five newly isolated alg-1 alleles carry missense mutations.

Figure 2

(A) A schematic showing the positions of the newly identified alg-1 mutations within the ALG-1 protein (black tics) and the existing deletion alleles (red lines). Positions of catalytic sites are indicated by yellow circles. (B) Exon/intron schematic of the two alg-1 isoforms predicted and supported by cDNA evidence (Wormbase.org). Boxes represent exonic regions. (C). Western blot analysis on total protein lysate from wild type and alg-1 mutant animals. All non-null alg-1 alleles, like the wild type, produce 2 isoforms of ALG-1. Newly identified missense alleles of alg-1 are marked in red and null alleles are in blue. alg-1(tm369) is a loss of function allele that deletes most of the PIWI domain and produces 2 truncated isoforms of ALG-1(*). All strains with the exception of alg-1(tm492) and alg-1(tm369) carry lin-31(lf) and col-19::gfp in the background.