Figure 9. Western blot analysis of ALG-1 immunoprecipitated complexes from extracts of alg-1(anti) and wild type animals.
Immunoprecipitated ALG-1(anti) shows an increased association with DCR-1, and a decreased association with AIN-1, compared to wild type ALG-1. The ratio of DCR-1 to ALG-1 and AIN-1 to ALG-1 were determined by quantitation of the Western blot signals, and each of those ratios for the alg-1(anti) mutants is normalized to that of the wild type. * means not applicable (n/a).