Table 2. lin-28(lf) phenotypes are suppressed by mutations in alg-1.
| Percentage of Animals with Adult Alae Synthesisa | Percentage of Animals that express col-19::gfp b | Average seam cell numberc | ||||||||
| L4 | L4 | L4 | ||||||||
| Genotypeb | No alae | Gapped | Comp. | (n) | No gfp | Seam only | Hyp+seam | (n) | (n) | |
| wild-type | 100 | 0 | 0 | 32 | 100 | 0 | 0 | 32 | 12.0 | 32 |
| lin-28(n947) | 0 | 0 | 100 | 32 | 0 | 6 | 94 | 32 | 8.1 | 32 |
| lin-28(n719) | 11 | 0 | 89 | 27 | 0 | 30 | 70 | 27 | 8.2 | 27 |
| lin-28(tm4566) | 20 | 18 | 62 | 91 | 2 | 57 | 41 | 64 | 8.2 | 64 |
| lin-28(n947); alg-1(ma198) | 70 | 20 | 10 | 30 | 0 | 70 | 30 | 30 | 10.6 | 30 |
| lin-28(n947); alg-1(ma192) | 100 | 0 | 0 | 31 | 0 | 100 | 0 | 31 | 10.7 | 31 |
| lin-28(n947); alg-1(ma195) | 100 | 0 | 0 | 32 | 0 | 100 | 0 | 32 | 9.7 | 32 |
| lin-28(n947); alg-1(ma202) | 97 | 0 | 3 | 30 | 0 | 97 | 3 | 30 | 10.9 | 49 |
| lin-28(n947); alg-1(ma203) | 87 | 10 | 3 | 31 | 0 | 100 | 0 | 31 | 9.5 | 30 |
| lin-28(n719); alg-1(ma203) | 64 | 36 | 0 | 14 | 7 | 93 | 0 | 14 | 10.2 | 13 |
| lin-28(tm4566); alg-1(ma202) | 68 | 32 | 0 | 25 | 24 | 75 | 0 | 25 | 9.2 | 17 |
| lin-28(n947); alg-2(ok304) | 3 | 9 | 88 | 34 | nd | nd | nd | nd | nd | nd |
| lin-28(tm4566); alg-2(ok304) | 19 | 14 | 67 | 37 | nd | nd | nd | nd | nd | nd |
a
Presence and quality of cuticular alae structures were assayed by Normarski DIC optics. Only one side of each animals was scored.
b
All animals contain maIs105 transgene which expresses an adult specific, col-19::GFP reporter.
c
Average number of seam cells per side was counted from pharynx to anus and therefore may contain 1 seam cell of the H lineage.
nd = not determined.