Skip to main content
. 2014 Apr 24;10(4):e1004306. doi: 10.1371/journal.pgen.1004306

Figure 5. The regulation of MST-1 and SID-1 by CDC-7 is based on distinct mechanisms.

Figure 5

(A) In vitro DBF-2 activity assays after addition of the indicated kinases. MST-1-stimulated DBF-2 activity is inhibited by addition of CDC-7 or CDC-7(D195A), while SID-1-stimulation requires active CDC-7. Western blot analysis of the precipitated proteins was used to determine comparable kinase levels (n = 4). (B) A GFP fusion construct of wt CDC-7 localized to spindle pole bodies and accumulated around the mature septal pore, while a CDC7(D195A)-GFP construct exclusively localized to SPBs. Nuclei were labeled with histone H1-RFP and plasma membrane with FM4-64.