Figure 6. SID-1 and POD-6 are pathway-specific activators of the SIN and MOR, while MST-1 regulates both NDR kinase pathways.

(A) In vitro kinase assays of precipitated MST-1 and SID-1 specifically stimulated DBF-2, but not DBF-2(T671A). (B) COT-1 was specifically phosphorylated by the upstream GC kinases POD-6 and MST-1, but not by SID-1. Western blot analysis of the precipitated proteins was used to determine comparable kinase levels.