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. 2014 Apr 24;10(4):e1004291. doi: 10.1371/journal.pgen.1004291

Figure 1. PCH-2's role in regulating synapsis is temperature sensitive.

Figure 1

A. Cartoon of the C. elegans germline divided into six zones of equal size. In all graphs, meiotic progression is from left to right. B. Meiotic nuclei in wildtype worms stained with antibodies against the axial element component HTP-3 (red) and the central element component SYP-1 (green). Most nuclei exhibit complete colocalization on HTP-3 and SYP-1. One nucleus (indicated by arrow) includes stretches of HTP-3 without SYP-1, indicating the presence of unsynapsed chromosomes. Grayscale images of HTP-3 and SYP-1 are also provided. Unless stated otherwise, scale bars in all images represent 5 microns. C. Histograms representing the percentage of nuclei that exhibit complete synapsis as a function of meiotic progression in wildtype and pch-2 mutant worms at 15°C (i), 20°C (ii) and 25°C (iii). Error bars indicate 95% confidence interval. For all graphs, a * indicates a p value<0.05 and a ** indicates a p value of <0.0001. Significance was assessed by performing Fisher's exact test.