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. 2014 Apr 24;10(4):e1004291. doi: 10.1371/journal.pgen.1004291

Figure 9. Mutation of pch-2 reduces the percentage of nuclei with six GFP::COSA-1 foci in meDf2 homozygotes.

Figure 9

A. A dissected germline stained with DAPI (blue) and antibodies against SUN-1 pSer8 (red) and PCH-2 (green). The extension of PCH-2 staining into late pachytene correlates with the extension of phosphorylated SUN-1 staining. Scale bar represents 20 microns. B. Histogram representing the percentage of meiotic nuclei that contain a given number of GFP::COSA-1 foci in meDf2 and meDf2;pch-2 mutants. 174 meDf2 nuclei and 178 meDf2;pch-2 nuclei were counted. Error bars represent 95% confidence interval. C. Histogram representing the average number of RAD-51 foci per nucleus in meDf2 and meDf2;pch-2 mutants. Error bars indicate standard error of the mean. For both graphs, a * indicates a p value<0.05. Significance was assessed by performing either Fisher's exact test (7B) or a paired t-test (7C). D. Genetic analysis of meiotic recombination in wildtype, pch-2, meDf2 and meDf2;pch-2 mutants represented as fractions of wildtype recombination and color-coded according to the legend on the right.