Figure 1. RAS1 and CDC24 are required for hypoxic growth in C. neoformans but not in S. pombe.

(A) Three-fold serial dilutions of each strain of C. neoformans were spotted on YEPD agar medium and incubated at 20% O₂, or 1% O₂ and 5% CO₂, or 5% CO₂ and 20% O₂ at 30°C for 3 days. (B) Cultures of S. pombe were spotted on YES medium and incubated at 30°C with 20% O₂ or with 0% O₂ for 3 days. (C) The indicated strains were spotted on YEPD agar medium and incubated at 20% O₂, or 1% O₂ and 5% CO₂ at 30°C for 3 days. For convenience, we only indicated the oxygen concentration without mentioning the CO₂ concentration. The name of each strain is indicated on the left.