Figure 3. Spot assay for growth phenotype.

Three-fold serial dilutions of each strain were spotted on YEPD medium and incubated at indicated conditions. Suppressor test in cdc24Δ (A) or ras1Δ (B). Two independent transformants of cdc24Δ and ras1Δ were tested. The cultures were incubated in 20% O₂ at 30°C for 3 days or in 1% O₂ at 30°C for 5 days. (C, D and E) Growth phenotype of various mutants. The cultures were incubated in 20% O₂ at 30°C for 3 days or in 1% O₂ at 30°C for 3 (C and E) or 5 days (D). Growth assays presented in each panel of the composite images of A–D were performed on the same day. (F) Phenotype of ras1Δcdc24Δ double deletant. The cultures were incubated either at 28°C, 37°C, 20% O₂ with 5% CO₂ at 28°C, or 5% O₂ with 5% CO₂ at 28°C for 3 days, respectively. (G) Phenotype of cdc24Δptp3Δ double deletant. The cultures were incubated either at 28°C, 37°C, 20% O₂ with 5% CO₂ at 28°C, or 6% O₂ without CO₂ at 28°C for 3 days, respectively.