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. 2014 Apr 24;8(4):e2823. doi: 10.1371/journal.pntd.0002823

Figure 4. CxTRAF, but not CxTRAF3, is required for activation of WNV-induced CxVago.

Figure 4

Hsu cells were transfected with dsRNA against TRAF-3 (TRAF-3 dsRNA) or TRAF (TRAF dsRNA). GFP dsRNA was used as a silencing control. At 24 h post-transfection, the cells were infected with WNV and total RNA was collected 48 hpi. (A) Real-time RT-qPCR was performed on uninfected cells using TRAF-3- or TRAF-specific primers. RpL32 primers were used as internal control. (B) Real-time RT-qPCR was performed using CxVago primers with RpL32 as control. (C) Real-time RT-qPCR was performed using WNV NS1 primers with RpL32 as control. (D) Viral titer estimation by plaque assays was conducted on the supernatant media from these cells at 48 hpi. Error bars represents standard error from three separate experiments with assays performed in triplicate (Student's t-test *p<0.05, compared to GFP dsRNA).