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. 2014 Apr 24;8(4):e2826. doi: 10.1371/journal.pntd.0002826

Figure 1. Enzymatic hydrolysis of Abz-LVEALYQ-EDDnp by Atr-I.

Figure 1

Cleavage of Abz-LVEALYQ-EDDnp by Atr-I results in a fluorescent emission dependent of the Atr-I enzymatic activity. Hydrolysis of the substrate was assessed after incubation of 11 ng of Atr-I alone (▾) or mixed with EDTA 2 mM (▪) with the FRET substrate for 30 minutes at 37°C. Abz-LVEALYQ-EDDnp alone was used as negative control (•).