Figure 5. Postexposure treatment of DOG4 RV-infected mice with TriGAS prevents the elevation of corticosterone associated with pathogenic RABV in brain and serum.
A) Mice were infected i.n. with 105 FFU of DOG4 and 4 hr later either mock treated with PBS or treated with 107 FFU of TriGAS. An additional group of uninfected, untreated mice served as a reference. Six mice per group were euthanized for each time point, and the levels of brain corticosterone were determined via LC-GC/MS-MS2 as described in Materials and Methods. B) Groups were identical to those in A), with one additional group of animals receiving only TriGAS. Ten mice per group were bled at each time point and serum corticosterone was measured by EIA as described in Materials and Methods. The results represent comparative levels of corticosterone (+/− SE) *p<.05, **p<.01, and ***p<.001, respectively.