Table 3. Comparison of various ethanol productions using pretreated corn stover as the substrate found in the literatures and in the current study.
Pretreated method | Fermentation method | Hydrolysis Temp. (°C) | Temp. in SSF (°C) | Pre-hydrolysis time (h) | Fermentation time (h) | Enzyme loadings | Ethanol concentration (g/L) | Reference |
LHW | Fed-batch S-SSF | 50 | 36 | 18 | 60 | Cellulase 30–35 FPU/g substrate | 39.4 | In this work |
Alkaline | SSF | - | 33 | - | 72 | Cellulase 20 FPU/g substrate, β-glucosidase 10 CBU/g substrate | 27.8 | [58] |
Dilute sulfuric acid | SHF | 45 | 35 | 72 | 72 | Cellulase 15 FPU/g substrate, β-glucosidase 9 CBU/g substrate | 30.6 | [59] |
Dilute phosphoric acid | SHF | 45 | 35 | 72 | 48 | Cellulase 15 FPU/g substrate, β-glucosidase 9 CBU/g substrate | 26.4 | [60] |
Fungal | SSF | - | 37 | - | 72 | Cellulase 10 FPU/g solid | 25.0 | [61] |