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. 2014 Apr 24;9(4):e95455. doi: 10.1371/journal.pone.0095455

Table 3. Comparison of various ethanol productions using pretreated corn stover as the substrate found in the literatures and in the current study.

Pretreated method Fermentation method Hydrolysis Temp. (°C) Temp. in SSF (°C) Pre-hydrolysis time (h) Fermentation time (h) Enzyme loadings Ethanol concentration (g/L) Reference
LHW Fed-batch S-SSF 50 36 18 60 Cellulase 30–35 FPU/g substrate 39.4 In this work
Alkaline SSF - 33 - 72 Cellulase 20 FPU/g substrate, β-glucosidase 10 CBU/g substrate 27.8 [58]
Dilute sulfuric acid SHF 45 35 72 72 Cellulase 15 FPU/g substrate, β-glucosidase 9 CBU/g substrate 30.6 [59]
Dilute phosphoric acid SHF 45 35 72 48 Cellulase 15 FPU/g substrate, β-glucosidase 9 CBU/g substrate 26.4 [60]
Fungal SSF - 37 - 72 Cellulase 10 FPU/g solid 25.0 [61]