Figure 5. Effects of MOF on NF-κB mediated reporter gene expression.

A) Dose-dependent inhibition of 5 h lipopolysaccharide (LPS)-induced (1 µg/ml) NF-κB dependent reporter gene transcription by 30 min. pre-incubation of in vitro differentiated U937 macrophages with MOF concentrations ranging from 0.05–5 µg/ml. One-way ANOVA was used to evaluate significant effects on reporter gene activity induced by MOF. Significant differences between single LPS treatment and LPS combination treatment with MOF concentrations ranging from 0.05–5 µg/ml were identified using Tuckey's multiple comparisons post-hoc test. B) NF-κB dependent reporter gene expression in in vitro differentiated U937 macrophages exposed for 4, 5 or 6 h to LPS (1 µg/ml), with or without 60 min. pre-incubation with 5 µg/ml MOF. Two-way ANOVA and Bonferroni's post-hoc test were used to determine significant time- and MOF concentration-dependent effects on LPS-induced reporter gene activity. C) U937-3xB-LUC monocytes were in vitro differentiated for 24 h with PMA (10 ng/ml) in the presence or absence of 5 µg/ml MOF. Upon replacement of the medium in the different settings, cells were exposed to LPS for 6 h. One-way ANOVA was used to examine significant MOF effects on reporter gene activity. Significant differences between single LPS treatment and LPS treatment following pretreatment with MOF were evaluated using Tuckey's multiple comparisons post-test. All values are means of three independent experiments, with standard deviations represented by vertical bars, * indicating statistically significant differences assessed as described accordingly (P<0.05).