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. 2014 Apr 24;9(4):e95527. doi: 10.1371/journal.pone.0095527

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© 2014 Milenkovic et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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DNA methylation of blood leukocyte gDNA of 5 volunteers was bisulfite treated and amplified by specific biotinylated primer sets for respectively a highly methylated CpG probe region of the CCR6 gene, a medium methylated CpG probe region of the PCDHB4 gene and a weakly methylated CpG probe region of the MAFB gene. DNA methylation intensities obtained from the different CpG probes by CpG pyrosequencing were plotted against the β-values (0<x<1, reflecting 0–100% DNA methylation) obtained with the Illumina 450K array platform. Curve fitting reveals significant correlation of results between both platforms.