Figure 1. Disruption of BSK1 membrane localization affects BSK1 and FLS2 association in Arabidopsis protoplasts. Co-immunoprecipitation of BSK1 G2A and FLS2 from Arabidopsis protoplasts transiently expressing BSK1 G2A-GFP and FLS2-FLAG before (−) or 10 min after (+) elicitation with 1 µM flg22 as indicated. FLS2-FLAG alone was used as a negative control. Total protein was subjected to immunoprecipitation with GFP antibody, followed by immunoblot analysis with anti-FLAG antibody. These experiments were repeated three times with similar results.