Figure 1. Expression and functional analysis of miR820 in cultivated rice and wild rice species. (A) Northern blot analysis of miR820 expression in AA and BB Oryza species. (B) Detection of miR820-cleaved OsDRM2 mRNA by RNA ligation-mediated 5′-RACE (upper panel) in AA and BB species. The same cDNA templates were used for PCR to amplify uncleaved OsDRM2 (middle panel) and OsACTIN (bottom panel) as controls. (C) The relative expression levels of pre-MIR820 measured by qRT-PCR in AA, BB and BBCC Oryza species and calculated by subtracting the values with RT reaction by the values without RT reaction, then normalized by OsACTIN. Values are means of triplicate experiments, with bars showing standard errors. Probes and primers are described in our previous work.⁷