Figure 3. EPP1 inhibits the mRNA and protein levels of HY5. (A) Immunobloting assay showing HY5 protein level in the epp1 mutants and Col wild type. (B) HY5 expression level by qRT-PCR. Relative expression was normalized to UBQ1 and data represent mean ± SD of three biological replicates. (C) EPP1 protein level in hy5 mutant and Col wild type. Seedlings were grown in the dark for 5 d in all assays. Staining with coomassie brilliant blue of the gel serves as a loading control in (A and C).