Figure 1. Thermotolerance of CSD2 transgenic plants. (A) CSD2 expression in transgenic plants expressing normal coding sequence of CSD2 or the miR398-resistant form of CSD2 (mCSD2) under the control of the 35S promoter (these transgenic plants are referred to as CSD2 transgenic plants hereafter). (B) Thermotolerance of wild-type (WT) and CSD2 transgenic plants. Three-week-old soil-grown seedlings were subjected to 0 (control) or 8 d (heat) at 37°C. (C) Shoot fresh weight of WT and CSD2 transgenic plants shown in (B). (D) Chlorophyll content of WT and CSD2 transgenic plants shown in (B). (E) Survival rates of flowers of separate batches of 1-mo-old of WT and CSD2 transgenic plants under heat stress (37°C for 0, 6 or 8 d). Data presented in (B–E) are from one representative individual transgenic line of each transgene. Error bars represent the standard deviation [n = 4 in (A); n = 50–80 in (C–E)]. One-way ANOVA (Tukey-Kramer test) was performed for data in (C–E) and statistically significant differences are indicated by different lowercase letters (p < 0.008).