Figure 7. Lung vascular wall is significantly thickened by chronic CCl₄-treatment.

A. Immunofluorescent detection of αSMA. Identification of αSMA-positive (red color) vessels in lung sections from control and CCl₄-treated mice. Nuclei are blue. Arrows represent magnified vessels from control and experimental animals in the lower panels. Scale bar  = 100 µm (upper panel) and 20 µm (lower panel). B. Quantification of arterial wall thickness. Wall thickness of arteries (20–100 µm in diameter) is markedly upregulated in lungs of CCl₄-treated mice. n = 3 animals/group; 80–100 vessels were examined in each animal. * p<0.001 CCl₄-treated group vs control group.