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. 2014 Apr 24;9(4):e96027. doi: 10.1371/journal.pone.0096027

Table 1. Primers used in the egl LAMP assay.

Primer 5′-3′ primer sequence Final primer conc. (μM) Primer position*
F3_RS_egl GAGCAACTACATCTATCCGTC 0.2 330–350
B3_RS_egl CATCAGCCCGAAGATGAC 0.2 637–654
FIP_RS_egl ACAGCTCGTTCGCGTCGACGACAGCGCGACCTACTA 2 354–371, 446–463
BIP_RS_egl GGTTCGTCAACGCCGTGAGATCACGTTGCCGTAGTAG 2 476–493, 540–558
FLoop_RS_egl GTTCATGCCCTTGTTCTTG 2 372–390
Bloop_RS_egl GCTCGATCCGCACAACTA 2 516–533

*Position of the primer on R. solanacearum strain GMI100 endoglucanase precursor (egl) gene (GenBank accession number DQ657595) sequence.