Table 1. Primers used in the egl LAMP assay.
Primer | 5′-3′ primer sequence | Final primer conc. (μM) | Primer position* |
F3_RS_egl | GAGCAACTACATCTATCCGTC | 0.2 | 330–350 |
B3_RS_egl | CATCAGCCCGAAGATGAC | 0.2 | 637–654 |
FIP_RS_egl | ACAGCTCGTTCGCGTCGACGACAGCGCGACCTACTA | 2 | 354–371, 446–463 |
BIP_RS_egl | GGTTCGTCAACGCCGTGAGATCACGTTGCCGTAGTAG | 2 | 476–493, 540–558 |
FLoop_RS_egl | GTTCATGCCCTTGTTCTTG | 2 | 372–390 |
Bloop_RS_egl | GCTCGATCCGCACAACTA | 2 | 516–533 |
*Position of the primer on R. solanacearum strain GMI100 endoglucanase precursor (egl) gene (GenBank accession number DQ657595) sequence.