Table 2. Analytical sensitivity of the egl LAMP assay.
| Concentration (cells/mL) | GBBC 1172 (Phylotype I) | RUN 30 (Phylotype IIA) | GBBC 729 (Phylotype IIB) | LMG 2296 (Phylotype III) | RUN 71 (Phylotype IV) |
| tp (min) | tp (min) | tp (min) | tp (min) | tp (min) | |
| 108 | 12.7 ±0.38 | 15.8 ±1.59 | 12.9 ±1.07 | 12.2 ±0.06 | 19.4 ±1.67 |
| 107 | 14.2 ±1.81 | 16.5 ±2.73 | 14.7 ±1.76 | 13.8 ±1.56 | 18.8 ±1.90 |
| 106 | 17.0 ±3.03 | 18.1 ±3.97 | 16.3 ±1.86 | 15.7 ±2.03 | 19.3 ±2.44 |
| 105 | 18.1 ±3.13 | 23.3a | 17.7 ±2.41b | 17.4 ±2.02 | 21.3a |
| 104 | 20.8 ±3.99 | 26.3a | 21.3a | 20.5 ±2.28 | - |
| 103 | - | - | - | - | - |
| 102 | - | - | - | - | - |
| Tm | 94.1 ±0.10 | 93.5 ±0.27 | 93.2 ±0.08 | 94.1 ±0.14 | 93.8 ±0.25 |
Data are means (±SD), calculated from three independent runs.
“-”: negative result (absence of signal).
a
, detected once out of three replicates.
b
, detected twice out of three replicates.
Data obtained on different R. solanacearum phylotypes samples, from boiled bacterial suspensions.