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. 2014 Apr 24;9(4):e95972. doi: 10.1371/journal.pone.0095972

Figure 4. miR393 is required for proper induction of AUX/IAA and GH3 genes.

Figure 4

Northern blots of RNAs from wt and mir393ab mutant plantlets treated or not with 10 µM 2,4-D for 8 h. Without treatments, IAA17 and IAA19 have higher steady state levels in mir393ab mutants than in wt plants. Treatment with 2,4-D for 8 h induces the expression of all genes tested. GH3.5 accumulates to similar levels in wt and mutants, GH3.2, IAA19, IAA29, IAA5 and IAA2 accumulate to higher levels in mir393ab mutants than in wt plants while GH3.6 and IAA17 accumulate to lower levels in mir393ab mutants than in wt plants.