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. 2014 Apr 24;9(4):e95972. doi: 10.1371/journal.pone.0095972

Figure 5. miR393 is required for proper degradation of AXR3-NT:GUS proteins.

Figure 5

Representative pictures of wt and mir393 mutant roots expressing the HSpro:AXR3-NT:GUS gene. Without treatments, the AXR3-NT:GUS fusion protein is more stable in mir393a-1, mir393b-1 and mir393ab mutants compared to wt plants. Short treatments with 10 µM auxin induces the degradation of AXR3-NT:GUS, while treatment with 20 µM auxinole or MG132 blocks the degradation of AXR3-NT:GUS. This shows that the SCF complexes and proteasome are functional in the mutants as well. Clearance of presumably high levels of endogenous AUX/IAA proteins by treatment with 2,4-D for 4 h restores the proper degradation of AXR3-NT:GUS.