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. 2014 Apr 24;9(4):e95965. doi: 10.1371/journal.pone.0095965

Table 3. Kinetic parameters of wild type and mutant cHAD enzymes.

	K _m (µM acetoacetyl-CoA)	V_max (nmol/s/µg enzyme)	k _cat (s⁻¹)	k _cat/K _m (µM ⁻¹s⁻¹)	n ^a
Wildtype	72.0±13.4	1.3±0.1	45	0.6	1.1±0.04^a
R204A	83.2±9.7	0.8±0.05	28	0.3	1.2±0.04^a
Y209A	50.4±11.0	0.5±0.06	17	0.3	1.1±0.03^a
R204A/Y209A	115.6±24.5	0.3±0.04	10	0.09	1.2±0.02^a

^a

Hill coefficent and the values are measured from fluorescence data.