Table II.
Sequence of quantitative RT-PCR primers and sequence-specific probes for target genes.
| Target gene | Primer sequence (5’→3’) |
|---|---|
| TS | |
| Sense | ATTTACCTGAATCACATCGAGCC |
| Antisense | TCGAAGAATCCTGAGCTTTGG |
| TaqManProbe | FAM-AAAATTCAGCTTCAGCGAGAACCCAGACC-TAMRA |
| DPD | |
| Sense | TCCCCAAAAGGCCTATTCCT |
| Antisense | TGCTCAATTCACCAAATGTTCC |
| TaqManProbe | FAM-CCATCAAGGATGTAATAGGAAAAGCACTGCAG-TAMRA |
| TP | |
| Sense | GAGTCTATTCCTGGATTCAATGTCATC |
| Antisense | CCCACGATACAGCAGCCC |
| TaqManProbe | FAM-CAGATGCAAGTGCTGCTGGACCAGG-TAMRA |
| OPRT | |
| Sense | ACTACACTAGAGCAGCGGTTAGAATG |
| Antisense | AACTGAACTCCTGGAGTCAAGTGA |
| TaqManProbe | FAM-TTCTGGCTCCCGAGTAAGCATGAAACC-TAMRA |
The probes were labeled with a reporter dye (FAM), situated at the 5’- end of the oligonucleotide, and a quencher dye (TAMRA), located at the 3’- end. RT-PCR, reverse transcription-polymerase chain reaction; TS, thymidylate synthase; DPD, dihydropyrimidine dehydrogenase; TP, thymidine phosphorylase; OPRT, orotate phosphoribosyl transferase.