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. 2014 Apr 24;9(4):e95932. doi: 10.1371/journal.pone.0095932

Figure 6. Expression of the different myosin heavy chain (MHC) isoforms and myogenic factors in the low weight (LW) and random bred control (RBC) quail lines.

Figure 6

Time point comparison of the neonatal MHC isoform using 2E9 monoclonal antibody (A), adult MHC isoform using AB8 monoclonal antibody (B), Pax7 (E), MyoD (F), and Myf-5 (I) between the LW and RBC quail lines (1 quail per each time point). Quail line comparison of neonatal MHC isoforms at 3 and 75 d post-hatch (C), adult MHC isoform at 28, 42, and 75 d post-hatch (D), Pax7 at 7 and 14 d post-hatch (G), MyoD at 0, 3, and 7 d post-hatch (H), and Myf-5 at 3 and 7 d post-hatch (J) (3 quail per each time point and each quail line). Expressions of myogenin mRNA in the two quail lines at 0, 3, 7, 14, 28, 42, and 75 d post-hatch (K). Total RNA was isolated from the pectoralis major muscle. Expressions of myogenin were measured by quantitative real-time PCR with avian ribosomal protein 13 (RPS13) as a control for normalization. Protein staining of the different quail lines at each time point (L; 1 quail per each time point) and at 0, 3, 7, 14, 28, 42, and 75 d post-hatch (M; 3 quail per each time point and each quail line). Gels used as a control of protein loading. Bars indicate standard errors. Level of significance: NS, not significant; * P<0.05; ** P<0.01; *** P<0.001.