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. 2014 Apr 19;20:644–653. doi: 10.12659/MSM.890091

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© Med Sci Monit, 2014

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License

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Neighbor-joining phylogenetic tree relating amino acid sequences of Hirudo medicinalis calreticulin (HmCalR) and calreticulins of selected species. Calreticulin amino acid sequences were chosen in the 200 highest similarities after a BLAST-P program obtained from the NCBI. The calreticulin sequences from the following species (including protein accession numbers) present a respective homology with HmCalR: Amblyomma cooperi (AAR29934) 70.5%, Ixodes scapularis (AAQ18696) 69.5%, Apis mellifera (XP_392689) 67.9%, Nasonia vitripennis (NP_001155151) 63.4%, Hydra magnipapillata (XP_002161300) 66.4%, Nematostella vectensis (XP_001640172) 77.1%, Eisenia andrei (ABI74618) 80%, Hyriopsis cumingii (AFR69202) 75.2%, Crassostrea gigas (BAF63639) 76.5%, Pinctada fucata (ABR68546) 75.7%, Salmo salar (ACI33338) 66.2%, Danio rerio (NP_956007) 68.8%, Tetraodon nigroviridis (CAG07986) 69.7%, Xenopus laevis (NP_001080765) 69.5%, Gallus gallus (AAS49610) 69.0%, Homo sapiens (NP_004334) 71.8%, Mus musculus (NP_031617) 72%, and Rattus norvegicus (NP_071794) 71.2%.