Figure 5.

Inhibitory effect of soluble CalR (sCalR) and rabbit polyclonal anti-human calreticulin antibodies on HmC1q-mediated leech microglia chemotaxis assays. From leech microglial cells incubated alone in Leibovitz medium (L-15), the vehicle (negative control migration =100%) was compared as a chemoattractant to recombinant rHmC1q (positive control) and to rHmC1q mixed with sCalR. The chemotactic effect of rHmC1q alone as chemoattractant was also evaluated on cells alone (L-15) or pre-incubated with either rabbit polyclonal anti-human CalR antibodies or rabbit IgG control. Asterisks denote that cell migrations of the indicated samples were significantly different (p<0.01) than those using rHmC1q alone on cells alone (L-15). All results were obtained from 3 independent experiments by condition.