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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Hepatology. 2014 Mar 31;59(5):1972–1983. doi: 10.1002/hep.26971

Fig. 3. Overexpression of SIRT1 sensitizes the liver and hepatocytes to BA-induced damage.

Fig. 3

(A) TUNEL assay on liver sections 48h after PH. (B) TUNEL assay on primary hepatocytes stimulated with DCA (100μM) for 24h (C) Primary hepatocytes incubated with DCFH-DA (Red) before and 6h after stimulation with DCA. (D) WB of whole protein extracts using pJNK Ab. (E) Inhibition of pJNK with SP600125 protected SIRT hepatocytes from DCA-induced ROS and apoptosis as shown by DCFH-DA and TUNEL assay. (n = 5 animals/time point; In vitro experiments were performed three times, each in duplicate).