Figure 2. Transcriptional profiling of MAMP-inducible genes in A. thaliana protoplasts transfected with SFI effector constructs.

(A–C) Relative gene expression for the flg22-inducible genes FRK1 and WRKY17 (A, B) and the housekeeping gene EF1α (C) was assessed by quantitative real-time polymerase chain reaction (qRT-PCR) 0, 1 and 3 h after protoplasts were exposed to flg22. Transcript levels of the analyzed genes were normalized to the levels of the Actin transcript. GFP was used as a negative and AvrPto as a positive control for suppression of gene expression. One representative independent experiment out of four is plotted. Data is presented as mean ± SEM from three technical replicates.