Table 1.

Effect of Washing on Ethanol Content

Treatment†	Ethanolint, mM	% of prior content
0 mM ethanol	0.8 mM ± 0.5 mM
171 mM ethanol (1% v/v)
5 sec wash	55.8 mM ± 2.1 mM
1 × 60 sec wash	26.6 mM ± 4.6 mM	47.7%
2 × 60 sec wash	11.4 mM ± 0.5 mM	42.9%

^†Chorionated embryos were incubated 1hr in 171mM ethanol prior to washing and analysis. N = 25 embryos/group, assayed in triplicate, and 3 groups/treatment.