Table 2.
Positions and sizes of genetic loci linked to host cytokine and chemokine response to Plasmodium yoelli infection.
| MS | Chr | Distance (cM) | Forward primer | Reverse primer | Physi. Posi | Length (bp) | Recomb. Progeny defining the loci | No. Can. Genes | |
|---|---|---|---|---|---|---|---|---|---|
| Py1238 | 7 | 17.8 | 5 ′ TATAATACCATGTGACATGT3 ′ | 5 ′ GCTTATACGAGATATTGTGA3 ′ | 385973 | ||||
| Py2061-1 | 7 | 20 | 5 ′ AAGGGAAGAAATTCGGCA3 ′ | 5 ′ TACTCTTTCACCTTTGCAA3 ′ | 440592 | ||||
| Py627 | 7 | 20 | 5 ′ ATACATATAAGCTGCTACAA3 ′ | 5 ′ TAATGTGTGCAAATATGTGA3 ′ | 501081 | 60489* | |||
| Py1798 | 7 | 22.2 | 5 ′ GCTTAGTGACTAAAATGTG3 ′ | 5 ′ GCATACTTGGAAAATTCCT3 ′ | 532584 | 146,611 | E890#3 | F239#4 | 38 |
| Py2179 | 9 | 28.1 | 5 ′ TGCTTTCATATTCTTCCAC3 ′ | 5 ′ TTGAAGAAGATAGGAGTAC3 ′ | 472715 | ||||
| Py1861 | 9 | 30.6 | 5 ′ GCATACACATATGTTGAGA3 ′ | 5 ′ ACGGATGGAAACATTACAA3 ′ | 402219 | ||||
| Py148 | 9 | 30.6 | 5 ′ AGATTCAAGTACAAGGTCA3 ′ | 5 ′ CTTTAGCTAGTCATCGTC3 ′ | 373266 | 28953* | |||
| Py806 | 9 | 32.7 | 5 ′ CTACTATTCTTTATTGTACC3 ′ | 5 ′ CAGATGAAGTAAAAGGCAC3 ′ | 324346 | 148,369 | F120#5 | F994#3 | 30 |
| Py189-2 | 10 | 6.4 | 5 ′ ATTGTTTGGGAATTAGCGA3 ′ | 5 ′ TGTATTCCCAGGTGTATC3 ′ | 696263 | ||||
| Py280 | 10 | 10.7 | 5 ′ ATTAAAGGCCTTATGCACA3 ′ | 5 ′ TTACATCATTAGTATTGGCA3 ′ | 790202 | ||||
| Py56 | 10 | 10.7 | 5 ′ AAGTTGAAATAACAATGCCA3 ′ | 5 ′ CAAAGTACGCAATCCTCA3 ′ | 793886 | 3684* | |||
| Py1829 | 10 | 17.6 | 5 ′ ATATGGAGAAAATTAGCGAA3 ′ | 5 ′ CTAACGCCAGACATAAAAA3 ′ | 1109682 | 413,419 | F245#l | G007#3 | 112 |
| Py1316-3 | 12 | 17.4 | 5 ′ ATTCTGCAGCGTATGATC3 ′ | 5 ′ TGTTTCTGCTCATACTAGA3 ′ | 1362004 | ||||
| Py437 | 12 | 19.6 | 5 ′ CATCACCAACTTGTATTTC3 ′ | 5 ′ CCATAATAAAGCAGTTCTC3 ′ | 1387382 | ||||
| Py743 | 12 | 19.6 | 5 ′ TAATATCTGGTCGTTATTCT3 ′ | 5 ′ ATTTAGCTATACAAATAGCC3 ′ | 1399469 | 12087* | |||
| Py2260 | 12 | 26.5 | 5 ′ TAGCAGCATATTCCTTGC3 ′ | 5 ′ ATGGCTTCTTCCAATAAGT3 ′ | 1765986 | 403,982 | E995#3 | G001#4 | 110 |
| Py2035 | 13 | 15.1 | 5 ′ TGTGTATATTGCAAAGGAC3 ′ | 5 ′ GTATACACTGTGATTCCTA3 ′ | 1121475 | ||||
| Py2123 | 13 | 19.4 | 5 ′ CATTCTTAGTCATGTCACA3 ′ | 5 ′ ATAATGGCAATAATAATGGC3 ′ | 1231545 | ||||
| Py2609 | 13 | 19.4 | 5 ′ CTCATATTTAAGTTTTTCTCT3 ′ | 5 ′ TAGGAAGACGATCTCGCT3 ′ | 1421935 | 190390* | |||
| Py1084 | 13 | 21.7 | 5 ′ GCATATCCAGTGACGCTA3 ′ | 5 ′ CAATCGATTTGGGTGCTA3 ′ | 1490422 | 368,947 | F995#4 | G001#5 | 96 |
MS, microsatellite names; Chr, chromosome; Distance (cM), genetic distance; Forward primer and Reverse primer, primers for the amplification of the mcirosatellites; Physi. Posi, physical positions of the first base of the forward primers; Length (bp), the fragment sizes of the linked loci with MS markers detecting crossovers resulting changes in LOD scores; Recomb. progeny, names of the progeny with crossovers defining the mapped loci; No. Can. Gene, numbers of candidate genes in the loci.
*
indicates the distance between the two markers with the highest LOD scores.