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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Radiat Environ Biophys. 2014 Jan 30;53(2):265–272. doi: 10.1007/s00411-014-0514-0

Figure 3. Automated Imaging and Scoring of γ-H2AX Fluorescence.

Figure 3

Paired images of DAPI-labeled nuclei (Panel A) and γ-H2AX foci (Panel B) captured using a 60x oil immersion objective integrated over the nuclear area (thin line) with the area covered by the thick line used for background subtraction (Panel C). Averaged plot from 30 donors of γ-H2AX fluorescence after 4 Gy of γ rays (Panel D). Error bars show ± SEM.