Table 2. A comparison of HepG2 cell recovery between the antibody-based approach and the ligand-based approach at different time intervals.

Time intervals	The antibody-based approach		The ligand-based approach		P
	Detected cell number	Recovery (%)	Detected cell number	Recovery (%)
0 hour	163±9	82±5	131±15	66±8	0.035
24 hours	161±10	81±5	110±21	55±11	0.019
48 hours	153±15	77±8	77±18	39±9	0.005
72 hours	136±28	68±14	38±24	19±12	0.010

NOTE: In the antibody-based approach, all observed differences between CTC values at 0 hour and after storage were not statistically significant (Student’s t-test).