Table 1. Comparative analysis of the 23 non-synonymous substitutions in protein-coding genes inferred in the full set of 30 sequences.

Position	Gene	Allelic variants	aa. replacement	Homologous aa. in rCRS	Variants in MITOMAP1	Occurrences in GB	MutPred pathogenicity score in human2	mtDNA selection score in human2
2767	nd1	T/C	F9L	L	L-L	23	0.625	0,768
4587	nd2	T/C	T227I	T	T-A	1	0.586	0.661
7053	coxII	A/G	T13A	T	T-T	122	0.378	0.297
7185	coxII	A/G	D57N	D	D-N, D-A, D-G, D-D	10, 1, 1, 3	0.499	0.473
8056	atp6	A/G	V43I	I	I-V, I-T, I-I	9, 5, 1127	0.325	0.242
8114	atp6	A/G	N62S	N	N-S	6	0.526	0.525
8134	atp6	A/G	T69A	S	T-T	7	n.a.	n.a.
8596	atp6	T/C	H223Y	H	H-R	3	0.656	0.865
8704	coxIII	T/C	I32T	A	A-V, A-A	8, 26	n.a.	n.a.
8731	coxIII	T/C	A41V	T	T-A, T-M, T-T	7, 1, 33	n.a.	n.a.
9015	coxIII	A/G	V136I	V	V-V	8	n.a.3	n.a.3
9112	coxIII	T/C	M168T	L	L-L, L-S	17, 1	n.a.	n.a.
9369	coxIII	C/G	V254L	V	V-I, V-V	117, 9	0.471	0.425
9548	nd3	T/C	V29A	G	G-S	66	n.a.	n.a.
10433	nd4	T/C	P89S	L	L-P, L-L	64, 4	n.a.	n.a.
10493	nd4	A/G	T109A	T	T-P, T-A, T-T	n.a., 109, 6	0.482	0.443
11872	nd5	A/G	C42Y	S	S-S	3	n.a.	n.a.
13100	nd5	A/T	I451M	L	L-L	5	n.a.	n.a.
13390	nd5	T/C	S548L	L	L-L	62	n.a.3	n.a.3
13964	nd6	A/G	V39A	V38	V-L, V-I	2, 6	0.544	0.562
14479	cytb	A/T	F109L	Y	Y-N, Y-H, Y-C	n.a., 33, 1	n.a.	n.a.
14795	cytb	A/G	T215A	S	S-S	19	n.a.	n.a.
14865	cytb	T/C	V238A	S	S-P, S-F, S-S	28, 7, 8	n.a	n.a.

¹Non synonymous variants are bolded. For positions in which only synonymous variants are known italics is used;

²from ref. [22];

³same aminoacid encoded by a different codon in rCRS and goat;

n.a.  =  not applicable.