Figure 10. Spatial distribution of Cy3-DNA foci after 15 min of exposure to transforming Cy3-DNA in ΔpilV and wt.
a) Typical examples of Cy3-DNA single cell fluorescence for different fragment lengths (ΔpilV). The white line denotes the cell contour as obtained from the bright field image. b) Two-dimensional distribution of focus location (ΔpilV). The color encodes the probability for finding a focus within the 2D bin. (N>18000 for each condition). c) Two-dimensional distribution 10% brightest foci (ΔpilV). d, e) Typical examples of Cy3-DNA single cell fluorescence for different fragment lengths (wt). f) Two-dimensional distribution of focus location (wt) (N = 309).