. 2014 Apr 24;10(4):e1004101. doi: 10.1371/journal.ppat.1004101

Table 2. Strains and plasmids used in this study.

Name	Relevant genotype	Origin/reference
Bacterial Strains
E. coli DH5α	φ80 dlacZΔM15Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (rk⁻. mk⁺) supE44 thi-1 gyrA relA1.	Laboratory stock
E. coli BL21 (DE3)	F– ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]).	Agilent Technologies
B. subtilis 168	trpC2.	Bacillus Genetic Stock Center
S. meliloti 1021	expR102::ISRm2011-1 expR. Sm^r.	[41]
L. crescens BT-1	Standard strain (Wild Type).	[13]
BS1	B. subtilis 168 ΔthrC:: [PCLIBASIA_01185 (−395 to +47)-lacZ]. Em^r.^a	This work
BS2	B. subtilis 168 ΔthrC:: [PCLIBASIA_01185-ldtR_Las (−395 to +516)-lacZ]. Em^r.^a	This work
BS3	B. subtilis 168 ΔthrC:: [PldtR_Las (−395 to +47)-lacZ]. Em^r.^a	This work
BS4	B. subtilis 168 ΔthrC:: [PldtR_Las-ldtR_Las (−395 to +516)-lacZ]. Em^r.^a	This work
BS5	B. subtilis 168 ΔthrC:: [PldtP_Las (+467 to +792)-lacZ]. Em^r.^a	This work
BS6	B. subtilis 168 ΔthrC:: [PldtR_Las-ldtR_Las-PldtP_Las (−395 to +792)-lacZ]. Em^r.^a	This work
BS6M1	BS6 [−111 AT→GG]. Em^r.^b	This work
BS6M2	BS6 [−108 TT→GG ]. Em^r.^b	This work
BS6M3	BS6 [−104 TT→GG ]. Em^r.^b	This work
BS6M4	BS6 [−102 GT→AG ]. Em^r.^b	This work
BS6M5	BS6 [−99 TtT→GtG ]. Em^r.^b	This work
SMP1	S. meliloti 1021 ldtP_Smc (+498)::uidA. Sm^r. Neo^r.	This work
SMP2	S. meliloti 1021 ldtR_Smc (+29)::uidA. Sm^r. Neo^r.	This work
SMP3	S. meliloti 1021 ldtP_Smc (+1332)::uidA. Sm^r. Neo^r.	This work
SMP2A	S. meliloti SMP2 pBBR1MCS-5. Sm^r. Neo^r. Gm^r.	This work
SMP2B	S. meliloti SMP2 pSMP4. Sm^r. Neo^r. Gm^r.	This work
SMP2C	S. meliloti SMP2 pSMP5. Sm^r. Neo^r. Gm^r.	This work
Plasmids
p15TV-L	Expression vector for purification of proteins by nickel affinity cromatography. Ap^r.	Structural Genomics Consortium, University of Toronto
pDG1663	B. subtilis vector for ectopic integration into thrC site containing E. coli spoVG-lacZ. Ap^r, Em^r.	[31]
pRK600	Helper plasmid for triparental mating. pRK2013 Nm::Tn9. Cm^r.	[35]
pVMG	pVO155 with stop codons upstream of uidA in all ORFs. Neo^r.	[42]
pBS1	P_{CLIBASIA_01185}-lacZ transcriptional fusion carrying ‘Ca. L. asiaticus’ sequence from −395 to +47 in pDG1663. Ap^r, Em^r.^a	This work
pBS2	P_{CLIBASIA_01185}-ldtR-lacZ transcriptional fusion carrying ‘Ca. L. asiaticus’ sequence from −395 to +516 in pDG1663. Ap^r, Em^r.^a	This work
pBS3	P_ldtR-lacZ transcriptional fusion carrying ‘Ca. L. asiaticus’ sequence from −395 to +47 in pDG1663. Ap^r, Em^r.^a	This work
pBS4	P_ldtR-ldtR-lacZ transcriptional fusion carrying ‘Ca. L. asiaticus’ sequence from −395 to +516 in pDG1663. Ap^r, Em^r.^a	This work
pBS5	P_ldtP-lacZ transcriptional fusion carrying ‘Ca. L. asiaticus’ sequence from +467 to +792 in pDG1663. Ap^r, Em^r.^a	This work
pBS6	P_ldtR-ldtR-P_ldtP-lacZ transcriptional fusion carrying ‘Ca. L. asiaticus’ sequence from −395 to +792 in pDG1663. Ap^r, Em^r.^a	This work
pBS6M1	pBS6 (−111) AT→GG. Ap^r, Em^r.^b	This work
pBS6M2	pBS6 (−108) TT→GG. Ap^r, Em^r.^b	This work
pBS6M3	pBS6 (−104) TT→GG. Ap^r, Em^r.^b	This work
pBS6M4	pBS6 (−102) GT→AG. Ap^r, Em^r.^b	This work
pBS6M5	pBS6 (−99) TtT→GtG. Ap^r, Em^r.^b	This work
pSMP1	Internal 394 bp of ldtP_Smc (from +104 to +498) in pVMG. Neo^r.^b	This work
pSMP2	407 bp of PldtR_Smc and ldtR_Smc (from −378 to +29) in pVMG. Neo^r.^a	This work
pSMP3	ldtP_Smc-gus transcriptional fusion in pVMG carrying S. meliloti sequence from +932 to +1332. Neo^r.^b	This work
pBBR1MCS-5	Broad host range vector. Gm^r.	[9]
pSMP4	ldtR _Las (from +1 to +516) cloned into pBBR1MCS-5 (EcoRI/BamHI). Gm^r.^a	This work
pSMP5	ldtP _Las (from +1 to +1296) cloned into pBBR1MCS-5 (KpnI/EcoRI). Gm^r.^b	This work

The positions indicated are relative to ldtR translational start site.

The positions indicated are relative to ldtP translational start site.