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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: J Neurochem. 2014 Feb 12;129(4):743–752. doi: 10.1111/jnc.12657

Figure 1. Sp4 is phosphorylated in response to resting membrane potential.

Figure 1

CG neurons were exposed to 25mM (depolarizing) or 5mM (resting) KCl for one hour. Protein extracts were treated with or without a protein phosphatase (PPase), separated by 6% PAGE or 10% PAGE and analyzed by Western blot using antibodies recognizing Sp4 and GAPDH as a loading control. Shown is a representative immunoblot of an experiment performed 5 times.