Skip to main content
. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: J Neurochem. 2014 Feb 12;129(4):743–752. doi: 10.1111/jnc.12657

Figure 6. A phospho-mimetic Sp4 S770D fails to rescue Sp4-knockdown dependent deficits in dendrite patterning in cerebellar granule neurons.

Figure 6

A. Extracts from Neuro2A cells cotransfected with control or Sp4 shRNA, along with RNAi-resistant FLAG-Sp4 wild-type (WT res), S770A (A res), or S770D (D res) and GFP were analyzed by Western blot. Results are a representative immunoblot from an experiment performed three times. B. Representative images of CG neurons transfected with the indicated constructs and analyzed by immunofluorescent microscopy for GFP. C. Histograms depicting the number of neurons with the indicated number of dendrites that were observed in each condition. D. Quantification of average primary dendrite numbers from the experiment described in (B). N=80–90, **p<0.01, ANOVA (F=12.01; p<0.0001).