FIG. 8.
Indomethacin-sensitive coronary artery responses to ET-1. Segments of the coronary artery were isolated from male rats 24 h following IT delivery of C60 or vehicle. Paired LAD segments isolated from each of the IT exposed male rats were also treated with 10μM Indomethacin 20 min prior to ET-1 administration. (A) Cumulative concentration-response curves developed in response to ET-1 revealed enhanced isometric stress generation in coronary arteries from C60 exposed rats when compared with vehicle. (B) Coronary segments isolated from C60 exposed rats showed sensitivity to Indomethacin during cumulative concentration responses to ET-1 when compared with vehicle. (C) Data combined from vehicle and C60 groups during ET-1/Indomethacin experiments showed that LAD isolated from vehicle instilled rats was not sensitive to Indomethacin during cumulative concentration responses to ET-1 and that Indomethacin restored LAD smooth muscle contractile response from IT C60 exposed rats to the level of those from the vehicle group. †p < 0.05 by regression analysis of best-fit curve values, *p < 0.05 by repeated measures ANOVA on matching concentration data points, N = 6.