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. Author manuscript; available in PMC: 2014 Apr 25.
Published in final edited form as: Cancer Discov. 2013 Jan 9;3(4):458–469. doi: 10.1158/2159-8290.CD-12-0464

Figure 3. NRASG12D induces melanocytosis in embryonic mice.

Figure 3

(A) Photograph showing a representative whole brain from an NrasLSL-G12D/LSL-G12D;Tyr::CreA/° (G12D/G12D) and control mouse at 3 weeks of age. Note the hyperpigmentation of leptomeninges following the gyri (black arrow) and sulci (black arrowhead) and the arboriform pattern over the parietal lobe (white arrow).

(B) Upper panels: photomicrographs of H&E stained mouse brains of control mice at 1 day and 3 weeks of age showing a single array of non-pigmented leptomeninges lining the cerebral parenchyma (black arrows). Scalebar = 50μm. Lower panel: photomicrographs of H&E stained mouse brains of NrasLSL-G12D/LSL-G12D;Tyr::CreA/° (G12D/G12D) mice at 1 day and 3 weeks of age showing hyperpigmentation and thickening of the leptomeninges (black arrows). Scalebar = 50μm. n = 6 mice / experimental group.

(C) Photomicrographs of an HMB45/MelanA stained mouse brain (arrows indicate individual cells) from an NrasLSL-G12D/LSL-G12D;Tyr::CreA/° (G12D/G12D) mouse at 1 day of age. Scalebar = 50μm. The boxed area in the left panel is shown in higher magnification in the right panel.