Table 1.
Proteomic analysis of proteins copurified with CATSPER1.
| Band number | Protein name | Accession No. | No. of peptides | Coverage (%) |
|---|---|---|---|---|
| Testis specific | ||||
| 9 | CATSPER1 | 21314844 | 8 | 19.7 |
| 8 | CATSPER2 | 124001554 | 4 | 7.9 |
| 3 | CATSPER3 | 30350218 | 6 | 17.6 |
| *4 | CATSPER4 | 29244466 | 7 | 18.5 |
| 16 | CATSPERβ | 257900479 | 34 | 39.3 |
| 14 | CATSPERγ | 157951745 | 7 | 8.3 |
| 12 | TMEM146 | 261245039 | 2 | 2.7 |
| 12 | Unnamed protein product (hypothetical protein LOC75721) | 34147101 | 2 | 3.1 |
| 11 | HSPA2 (HSP70-2) | 123621 | 15 | 32.9 |
| Not testis-specific | ||||
| *13 | PARASPECKLE | 225543409 | 8 | 14.1 |
| 12 | SLC39A6 | 26324938 | 4 | 7.1 |
| *11 | GRP78 | 1304157 | 17 | 34.4 |
| 8 | Non-POU-domain-containing, octamer binding protein | 12963531 | 13 | 12.6 |
| 7 | Cleavage and polyadenylation specificity factor subunit 7 | 24416453 | 14 | 21.6 |
| *6 | Tubulin β-2c | 22165384 | 4 | 10.4 |
| *5 | SLC39A7 | 6680147 | 3 | 15.9 |
| *5 | Elongation factor Tu | 556301 | 4 | 8.4 |
| *2 | Cleavage and polyadenylation specificity factor subunit 5 | 13386106 | 15 | 90.3 |
*
Proteins identified from only one of two purifications.
Proteins verified to be in the CATSPER1 complex are in bold. Proteins tested but not found to be in the CATSPER1 complex are listed in italics. All other proteins were not further characterized.